Composite

Part:BBa_K741018:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-08-29)

plac-RBS-cI-T-pRM-anticro-T-pCon(0.856)-RBS-crogfp-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1589
    Illegal NheI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1370
    Illegal BglII site found at 1706
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2500


Design Notes

The fusion protein crogfp downstream the constitutive promoter BBa_J23102 is continuously expressed. If IPTG exists, the cI will be expressed and then activates the pRM to produce the antisense RNA of cro(anticro). The anticro will repress the expression of crogfp.

Source

BBa_K741001 BBa_K741009 BBa_K741016

References